PAKISTAN SOCIETY OF PHARMACOGNOSY & NATURAL THERAPY
PAKISTAN SOCIETY OF PHYTOPHARMACOLOGY & THERAPEUTICS
PAKISTAN SOCIETY OF PHARMACOGNOSY & NATURAL THERAPY*** PAKISTAN SOCIETY OF PHYTOPHARMACOLOGY & THERAPEUTICS
 
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IN VITRO INHIBITION OF UREASE AND a-CHYMOTRYPSIN BY
SOME SELECTED INDIGENOUS MEDICINAL PLANTS


Mansoor Ahmada, Taous Khana,b*, Muhammad Nisar\ Muhammad ArifLodhiC, Waqar Ahmada and Manzoor Ahmadc "Research Institute of Pharmaceutical Sciences, Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi-75270, Pakistan bDepartment of Pharmacy, University of Peshawar, Peshawar-25120, Pakistan ''H. E. J. Research Institute of Chemistry,
University of Karachi, Karachi-75270, Pakistan

 

ABSTRACT: The ethanol extracts derived from selected medicinal plants of Pakistan including Trichodesma indicum (whole plant), Paeonia emodi (Aerial parts), Aconitum leave (aerial parts) and Sauromatum gattatum (rhizomes, leaves and fruits) were screened' for enzyme inhibition activities against Urease
(J.B. and B.P.) and a-Chymotrypsin enzymes. Only extract derived from Paeonia emodi (Aerial parts) showed significant activity again Jack bean and B.P. Urease by inhibiting them by 74% and 80% respectively. On the basis of the significant inhibition of the.said enzyme from the two respective sources, the ethanolic extract of Paeonia emodi was further fractionated into n-hexane, CHCl" EtOAc, BuOH and H2O fractions and were tested for urease enzyme from both the sources. Significant inhibitory activity was observed in EtOAc, BuOH and H2O fractions while the n-hexane and CHCI3 fractions were devoid of any such activity. During a-Chymotrypsin enzyme inhibition studies by the selected medicinal plants the extracts derived from Trichodesma indicum (whole plant), Paeonia emodi (Aerial parts) and Sauromatum gattatum (leaves) showed inhibitory activity of the said enzyme. The derived fractions from Paeonia emodi (Aerial parts) where tested for this enzyme and the maximum activity was concentrated into EtOAc fraction.

KEYWORDS: Trichodesma indicum, Paeonia emodi, Aconitum laeve, Sauromatum gattatum, Urease, a-Chymotrypsin