PAKISTAN SOCIETY OF PHARMACOGNOSY & NATURAL THERAPY
PAKISTAN SOCIETY OF PHYTOPHARMACOLOGY & THERAPEUTICS
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COMPARISON OF THE EFFECTS OF AZALASTINE AND VERAPAMIL IN THE
MODIFICA TION OF BRONCHO-CONSTRICTION OF OVALBUMIN SENSITIZED
LUNG PARENCHYMAL TISSUES OF GUINEA PIGS IN VITRO

Syed Saud Hasanl, Aftab Turabe, Jan Mohammad Sheikh2, Mohammad Yousuf Salae and Mehar Ale I Department of Phannacology, Dow University of Health Sciences, Karachi, Pakistan 2NPCC, Jinnah Postgraduate Medical Centre,
Karachi, Pakistan


ABSTRACT: Pharmacological prevention of antigen-antibody mediated reaction can be accomplished, at least in principle either to Prophylaxis achieved by inhibiting the release of chemical mediators andlor blocking the tissue receptors that served as target of the mediator action. The ability of azalastine and verapamil to influences antigen induced contractile responses in isolated sensitized parenchymal tissues of Guinea pig in vitro. The Guinea pigs (n= I 0) were sensitized with ovalbumin and their parenchymal strips exposed to different concentration of . ovalbumin to calculate the ECso. Each sensitized parenchymal strips treated with either Azalastine or verapamil in an organ bath for 10 minutes and treated with ECso ovalbumin and contraction recorded by Grass Polygraph model 78. ECso (n=6) of parenchymal strips O.3x 10.6 + 0.16x1O.6g/ml and give a mean response of contraction (n=6) 9+0.44mm Azalastine in concentration 10.9 glml does not show any inhibitory effects but as the concentration increases to 10.8 glml marked inhibition recorded and at concentration 10.7 glml completely antagonizes the ECso induced contraction. While verapamil does not show any inhibition at concentration 10.10 glml and concentration 10.8 glml showed complete antagonism. It is concluded that inhibition of ovalbumin-induced contraction of sensitized parenchymal tissues of Guinea pig in vitro is dose dependent and controlled better with vrepamil than Azalastine.

KEY WORDS: Ovalbumin, Azalastine, Verapamil, Guinea pig.