PAKISTAN SOCIETY OF PHARMACOGNOSY & NATURAL THERAPY
PAKISTAN SOCIETY OF PHYTOPHARMACOLOGY & THERAPEUTICS
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PHARMACOKINETICS DIFFERENCES OF TAB MEFENAMIC ACID (MA) 5OOmg (ONE PILL) AND 250mg (TWO PILL) WITH AND WITHOUT FOOD

Obaid AliI, Roohi ObaidI, Noor Kamif, Zafar Saied Saify2, and Syed Waseemuddin Ahmed3 Ministry of Health, Government of Pakistan, Karachi, Pakistan department of Pharmaceutical Chemistry, Faculty of Pharmacy, University
of Karachi, Karachi-75270, Pakistan 3Department of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Karachi- 75270, Pakistan


ABSTRACT: Administration of two tab instead of one double strength tab is more often ordered by the physicians, pharmacists and even selected by the patients in case of over the counter drugs. In order to determine the ultimate quality of any formulated dosage form and rationalize the therapeutic plan as well as to individualize the prescription, in vivo measurement of drug is the modern and specialized expertise of the clinical I research area of pharmacy practice, which provides effectiveness and assures the safety of drugs. All pharmacological, therapeutic or toxic responses are subject to reaching of drug at the site of action through connective tissue. Other than physico-chemical properties of drug, there are numerous factors from manufacturing process to biochemical behaviour of the individual, which resist in the absorption, distribution, metabolism and elimination of drugs in the biological system. Tab MA Test II (250 mg) & Test I (500 mg) conventional formulations manufactured by local industries were investigated for
bioavailability followed by pharmacokinetic studies on adult, male, healthy, human local population. A sensitive, specific and validated method was developed for the estimation of MA in blood. HPLC was performed on a reversed phase CIs column (flow rate 1.0 ml/min, UV= 280 nm) with 10 mM buffer of KHzP04 (adjusted pH 2.9 with Phosphoric Acid) and Acetonitrile (70:30) where as extraction of the drug
from the plasma was carried out by deproteinization of plasma according to classical method described by Roohi Obaid et ai. 2002. Oral administration of MA Tablet Test I (single tab of 500 mg) and Test II (two pills of 250mg) with and without food to 12 healthy human volunteers was conducted. Peak level (T max) of MA tablet 500 mg (single tab) and 250 mg (two pilIs) of was observed at about 105 and .240 minutes without and with food respectively. Two pills of MA; Test II showed significantly increase the AUC and Cmax value in both cases either drug is administered with food or without food.

Keywords: Mefenamic acid, Bioavailability, OTC, Non-steroidal anti-inflammatory.